Nicaragua) and the Gruta del Espírito Santo (El Salvador). of Guatemala there are only two rock art caves (Bombil Pec and Cueva del Instituto Hondureño de Antropología e História (IHAH), htm .. Batres A., Carlos; Ramiro Martínez; Nury de Milián and Lucrecia Pérez. Egan, Muireann; Jiang, Hao; O’Connell Motherway, Mary; Oscarson, Stefan Integrando la historia clínica ambiental en el consejo prenatal y cuidado de 2 casos de Martínez, Noelia; Luque, Roberto; Milani, Christian; Ventura, Marco; Vieites, José María; Genovés, Salvador; Maldonado, José; Bermúdez-Brito. Domingo, María de la O, Oscar, Lina, Lariza, Gianluca, Daniele, Matteo,. Ramón, Alma, Mario y magmas contaminados por la corteza continental (Pearce y Peate, ;. Turner et al. .. tasa de exhumación e historia de enfriamiento del plutón, empleando una sedimentary sequence from San Salvador– Patlanoaya.
Ventriculoperitoneal shunt infection caused by Bifidobacterium salvwdor. Bifidobacterium breve is a rare cause of human infections. Previously, bacteremia and meningitis caused by this organism linked to probiotic use have been reported in a neonate.
We report the first case of a ventriculoperitoneal shunt infection caused by B. Cellodextrin utilization by bifidobacterium breve UCC Cellodextrins, the pexte hydrolysis products from insoluble cellulose, are accessible as a carbon source to certain members of the human gut microbiota, such as Bifidobacterium breve UCC Phenotypic analysis of a B.
Moreover, our results suggest that transcription of the cld cluster is controlled by a LacI-type regulator encoded by cldR, located immediately upstream of cldE. Gel mobility shift assays using purified CldR His produced by the incorporation of a His 12 -encoding sequence into the 3′ end of the cldC gene indicate that the cldEFGC promoter is subject to negative control by CldR Hiswhich binds to two inverted repeats.
The cldC gene of the cld operon of B. Comparative genomics of the Bifidobacterium breve taxon. Bifidobacteria are commonly found as part of the microbiota of the gastrointestinal tract GIT of a broad range of hosts, where their presence is positively correlated with the host’s health status. In this study, we assessed the genomes of thirteen representatives of Bifidobacterium brevewhich is not only a frequently encountered component of the adult and infant human gut microbiota, but can also be isolated from human milk and vagina.
In silico analysis of genome sequences from thirteen B. These latter genes specify the biosynthetic machinery for sortase-dependent pili and exopolysaccharide production, as well as genes that provide protection against invasion of foreign DNA i. Gene-trait matching analysis showed clear correlations between known metabolic capabilities and characterized genes, and it also allowed the identification of a gene cluster involved in the utilization of the alcohol-sugar sorbitol.
Genome analysis of thirteen representatives of the B. For this reason our analyses suggest that this number of B. Comparative genomics also facilitated the genetic explanation for differential carbon source utilization phenotypes previously observed in different strains of B. Discovery of a conjugative megaplasmid in Bifidobacterium breve. Bifidobacterium breve is a common and sometimes very abundant inhabitant of the human gut. Genome sequencing of B. In silico characterization of this element revealed several genomic features supporting a stable establishment of the megaplasmid mwrtinez its host, illustrated by predicted CRISPR-Cas functions that are known to protect the host against intrusion of foreign DNA.
Interestingly, pMP is also predicted to encode a conjugative DNA transfer apparatus and, consistent with this notion, we demonstrate here the conjugal transfer of pMP to representative strains of B. We also demonstrate the presence of a megaplasmid with homology to pMP in three B. Discovery of a Conjugative Megaplasmid in Bifidobacterium breve.
Blooms of the dinoflagellate Gymnodinium breve can produce sufficient concentrations histoia brevetoxin to negatively impact coastal ecosystems, human health, and local economies.
Assessment of risks associated with G. Bifidobacterium breve is an actinobacterium frequently isolated from colonic microbiota of breastfeeding babies. Oscqr, we report the complete and annotated genome sequence of a B.
The genome sequence will provide new insights into the biology of this potential probiotic organism and will allow the characterization of genes related to beneficial properties.
Bifidobacterium breve strains are numerically prevalent among the gut microbiota of many healthy breastfed infants. In the present study, we investigated lscar activity in a bacterial isolate from a nursling stool sample, B. Two putative sulfatases were identified on the genome of B. By using a combination of transcriptomic and functional genomic approaches, a gene cluster designated ats2 was shown to be specifically required for GlcNAcS metabolism.
Transcription of the ats2 cluster is regulated by a repressor open reading frame kinase ROK family transcriptional repressor. This study represents the first description of glycosulfatase activity within the Bifidobacterium genus. Bifidobacterium breve strains utilize a variety of plant- and host-derived carbohydrates that allow them to be present as prominent members of the infant gut microbiota as well as salvadir present in the gastrointestinal tract of adults.
In this study, we introduce a previously unexplored area of carbohydrate metabolism in bifidobacteria, namely, the metabolism of sulfated carbohydrates.
martniez GlcNAcS can be found in terminal or branched positions of mucin oligosaccharides, the glycoprotein component of the mucous layer that covers the digestive tract. Bifidobacteria constitute a specific group of commensal bacteria typically found in the gastrointestinal tract GIT of humans and other mammals.
Bifidobacteria are saccharolytic organisms naturally found in the digestive tract of mammals and insects.
The results of this study provide. Bifidobacteria constitute a specific group of commensal bacteria that inhabit the gastrointestinal tracts of humans and other mammals. Bifidobacterium breve UCC has previously hstoria shown to utilize several plant-derived carbohydrates that include cellodextrins, starch, and galactan. In the present study, we investigated the ability of this strain to utilize the mucin- and human milk oligosaccharide HMO -derived carbohydrate sialic acid.
Using a combination of transcriptomic and functional genomic approaches, we identified rl gene cluster dedicated to the uptake and metabolism of sialic acid. Furthermore, we demonstrate that B. Metabolism of sialic acid by Bifidobacterium breve UCC Transposon mutagenesis in Bifidobacterium breve: Bifidobacteria are claimed to contribute positively to human health through a range of beneficial or probiotic activities, including amelioration of gastrointestinal and metabolic disorders, and therefore this particular group of gastrointestinal commensals has enjoyed increasing industrial and scientific attention in recent years.
However, the molecular mechanisms underlying these probiotic mechanisms are still largely unknown, mainly due to the fact that molecular tools for bifidobacteria are rather poorly developed, with many strains lacking genetic accessibility. In this work, we describe the generation of transposon insertion mutants in two bifidobacterial strains, B.
Turning a Blind Eye
We also report the creation of the first transposon mutant library in a bifidobacterial strain, employing B. The library was found to be composed of clones containing single transposon insertions which appear to be randomly distributed along the genome. The usefulness of the library to perform phenotypic screenings was confirmed through identification and analysis of mutants defective in D-galactose, D-lactose or pullulan utilization abilities.
Probiotic Bifidobacterium breve induces ILproducing Tr1 cells in the colon. However, it remains unclear how mrtinez of another regulatory T cell subset, Tr1 cells, is regulated in the intestine. Here, we analyzed the role of two probiotic strains of intestinal bacteria, Lactobacillus casei and Bifidobacterium breve in T cell development in the intestine.
These findings demonstrate that B.
Bacteremia induced by Bifidobacterium breve in a newborn with cloacal exstrophy. Bifidobacterium breve is an effective probiotic agent used in the ek of neonatology. The pathogenic mechanism underlying the bacteremia is unknown. Herein, we report a second case of B. Following surgical repair immediately after birth, B. The bacteremia was thought to have been associated with an intestinal obstruction.
A pediatric culture bottle is theoretically unsuitable for incubating B. It was, however, cultured from pediatric culture bottles in the present case, suggesting that pediatric culture bottles may be useful for procuring B.
Blooms of the dinoflagellate Gymnodinium breve i. Characterizing and predicting bloom events and their impacts requires knowledge of G. We previously showed that microbial metabolism in the gut influences the composition of bioactive fatty acids in host adipose tissue. This study compared the effect of dietary supplementation for 8 wk with human-derived Bifidobacterium breve strains on fat distribution and composition and the composition of the gut microbiota in mice.
Tissue fatty acid composition was assessed by gas-liquid chromatography while 16S rRNA pyrosequencing was used to investigate microbiota composition. Visceral fat mass and brain stearic acid, arachidonic acid, and DHA were higher in mice supplemented with B.
The response of fatty acid metabolism to administration of bifidobacteria is strain-dependent, and strain-strain differences are important factors that influence modulation of the gut microbial community by ingested microorganisms. Bifidobacterium breve attenuates murine dextran sodium sulfate-induced colitis and increases regulatory T cell responses. While some probiotics have shown beneficial effects on preventing or treating colitis development, others have shown no effects.
In this study, we have assessed the immunomodulating effects of two probiotic strains, Lactobacillus rhamnosus L. With respect to the latter, the mRNA expression of T cell subset-associated transcription factors and cytokines in the colon was measured and the T helper type Th 17 and regulatory T cell Treg subsets were determined in the Peyer’s patches. In vivo intervention with B. In addition, intervention with B. Safety of Bifidobacterium breve BBG in preterm infants.
Sinceviable cells of Bifidobacterium breve BBG have been used in pediatric patients for clinical conditions such as intractable infantile diarrhea, preterm status, and pediatric surgery. Although the efficacy of BBG has been widely reported, adverse events related to the use of BBG have been reported in very few cases.
In order to trace adverse events seen by doctors in 88 medical institutions where BBG was used, a questionnaire survey of the number of occurrences and details of each case was conducted. Eighty-six clinicians 70 institutions responded to the questionnaire response rate, Number of respondents according to department of diagnosis no.
BBGtreated infants was as follows: Adverse events occurred in two extremely premature infants with functional ileus due to starch aggregates as vehicle, and in two surgical neonates with bacteremia caused by B. Adverse events related to the use of BBG have an extremely low incidence and are mild in severity, thus ensuring the superior safety of this preparation.
Capability of the two microorganisms Bifidobacterium breve B and Bifidobacterium breve BR03 to colonize the intestinal microbiota of children. The total number of bacteria present in the gut microbiota of a newborn is consistently lower than the average found in adults, with the extent of this difference being directly related to body weight and age.
It could be assumed that a lower number of viable probiotic cells is necessary to achieve significant gut colonization in infants and children. This study hiistoria the capability of Bifidobacterium breve B DSM and Bifidobacterium breve BR03 DSM2 strains able to mzrtinez inhibit some gram-negative bacteria in vitro, to integrate into the intestinal microbiota of children. Ten healthy children aged an average of 5. The daily dose was million live cells of each strain. Fecal specimens were collected and analyzed at the beginning d0 and at the end of the study d Total fecal bifidobacteria and coliforms have been quantified by microbiological plate counts.
A significant increase in total fecal bifidobacteria from 8.
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